Erythropoietin/EPO Active Protein | EPO active protein

Recombinant Mouse Erythropoietin/EPO (C-6His)

Cat. Num. AAA177969

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Erythropoietin/EPO; N/A; Recombinant Mouse Erythropoietin/EPO (C-6His); Erythropoietin; Epoetin; EPO; EPO active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Ala27-Arg192

• Species: Mouse
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala27-Arg192 is expressed with a 6His tag at the C-terminus.
• Activity: Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.35ng/mL.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

Bioactivity

SDS-PAGE

Related Product Information for EPO active protein

Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy.

NCBI and Uniprot Product Information

• NCBI GI #: 21389309
• NCBI GeneID: 13856
• NCBI Accession #: NP_031968.1
• NCBI GenBank Nucleotide #: NM_007942.2
• UniProt Accession #: Q0VED9; P07321
• Molecular Weight: 48,700
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: Q0VED9_MOUSE