Human beta-NGF CLIA Kit | beta-NGF clia kit

beta-NGF (Human) LumiAb ELISA Kit

Cat. Num. AAA318522

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Reactivity: Human
• Synonyms: beta-NGF; N/A; beta-NGF (Human) LumiAb ELISA Kit; Human beta-NGF; Beta-nerve growth factor; beta-NGF clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Betacellulin ELISA is capable of recognizing both recombinant and naturally produced Human Betacellulin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 241
• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for beta-NGF clia kit

Principle of the Assay: The LumiAb Human Betacellulin Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Betacellulin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Betacellulin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human Betacellulin is a 48 amino acid cytokine protein that is encoded by the BTC gene on chromosome 4 at locus 4q13-q21. The protein Probetacellulin is a 178 residue transmembrane precursor peptide that undergoes proteolytic cleavage, resulting in the mature form of Betacellulin. It has been determined that Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells via its high affinity interaction with the EGF receptor as it contains an EGF-like domain. The small peptide is expressed in several tissue types-predominantly in the pancreas, small intestine and range of tumor cells. The secondary structure of Betacellulin contains a 3 residue alpha helix and 5 beta strands composed of 18 residues.

Product Categories/Family for beta-NGF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 90110037
• NCBI GeneID: 4803
• UniProt Accession #: P01138
• NCBI Official Full Name: Beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF
• UniProt Entry Name: NGF_HUMAN