Human FGF-9 CLIA Kit | FGF-9 clia kit

FGF-9 (Human) LumiAb ELISA Kit

Cat. Num. AAA318592

• Gene Names: FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• Reactivity: Human
• Synonyms: FGF-9; N/A; FGF-9 (Human) LumiAb ELISA Kit; Human FGF-9; Glia-activating factor; GAF; Fibroblast growth factor 9; Heparin-binding growth factor 9; HBGF-9; FGF-9 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Fetuin A ELISA is capable of recognizing both recombinant and naturally produced Human Fetuin A proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Sequence Length: 208
• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for FGF-9 clia kit

Principle of the Assay: The LumiAb Human Fetuin A Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Fetuin A concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Fetuin A while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Fetuin A/AHSG promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone and therefore shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Fetuin A is synthesized in the liver and is selectively concentrated in bone matrix. It is also secreted in plasma and found in dentin in much higher quantities than other plasma proteins. Phosphorylation sites are present in the extracellular medium. There are two common alleles, AHSG*1 and AHSG*2-AHSG*1 has Thr-248/Thr-256; AHSG*2 has Met-248/Ser-256.

Product Categories/Family for FGF-9 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4503707
• NCBI GeneID: 2254
• NCBI Accession #: NP_002001.1
• NCBI GenBank Nucleotide #: NM_002010.2
• UniProt Accession #: P31371
• NCBI Official Full Name: fibroblast growth factor 9
• NCBI Official Synonym Full Names: fibroblast growth factor 9
• NCBI Official Symbol: FGF9
• NCBI Official Synonym Symbols: GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• NCBI Protein Information: fibroblast growth factor 9
• UniProt Protein Name: Fibroblast growth factor 9
• UniProt Gene Name: FGF9
• UniProt Synonym Gene Names: FGF-9; GAF; HBGF-9
• UniProt Entry Name: FGF9_HUMAN