Human Programmed Cell Death Protein 1 Ligand 1 (PDL1) CLIA Kit | PDL1 clia kit

Human Programmed Cell Death Protein 1 Ligand 1 (PDL1) CLIA Kit

Cat. Num. AAA188983

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: CLIA (Chemiluminescence Immunoassay)
• Synonyms: Programmed Cell Death Protein 1 Ligand 1 (PDL1); N/A; Human Programmed Cell Death Protein 1 Ligand 1 (PDL1) CLIA Kit; Programmed Cell Death Protein 1 Ligand 1; CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1; PDL1 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 1 Ligand 1 (PDL1).; No significant cross-reactivity or interference between Programmed Cell Death Protein 1 Ligand 1 (PDL1) and analogues was observed.
• Sequence Length: 176
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.5pg/mL

• Applicable Applications for PDL1 clia kit: CLIA (Chemiluminescence Immunoassay)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Programmed Cell Death Protein 1 Ligand 1 (PDL1) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NP_001254635.1
• UniProt Accession #: Q9NZQ7
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN