FCM/FACS (Flow Cytometry)
(Figure 3. Flow cytometry analysis with 1ug/mL Canine PD-L1 Protein, hFc Tag(AAA129507) on Expi293 cells transfected with Canine PD-1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
PD-L1 Recombinant Protein | PDL1 recombinant protein
Canine PD-L1 Protein, hFc Tag
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
PD-L1; N/A; Canine PD-L1 Protein, hFc Tag; B7-H; B7H1; CD274; PDCD1L1; PDCD1LG1; PDL1; hPD-L1; Recombinant Canine PD-L1 protein with C-terminal human Fc tag; PDL1 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Sequence
PD-L1 (Phe19-His238) hFc (Glu99-Ala330)
Tag
C-Human Fc
Preparation and Storage
Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
FCM/FACS (Flow Cytometry)
(Figure 3. Flow cytometry analysis with 1ug/mL Canine PD-L1 Protein, hFc Tag(AAA129507) on Expi293 cells transfected with Canine PD-1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
FCM/FACS (Flow Cytometry)
(Figure 3. Flow cytometry analysis with 1ug/mL Canine PD-L1 Protein, hFc Tag(AAA129507) on Expi293 cells transfected with Canine PD-1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, mFc Tag can bind Canine PD-L1 Protein, hFc Tag (AAA129507) in a linear range of 16–2000 ng/mL.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, mFc Tag can bind Canine PD-L1 Protein, hFc Tag (AAA129507) in a linear range of 16–2000 ng/mL.)
SDS-PAGE
(Figure 1. Canine PD-L1 Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Canine PD-L1 Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for PDL1 recombinant protein
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Product Categories/Family for PDL1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
The protein has a predicted molecular mass of 51.1kDa after removal of the signal peptide.
Similar Products
Product Notes
The PDL1 (Catalog #AAA129507) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: PD-L1 (Phe19-His 238) hFc (Glu99-Ala 330). It is sometimes possible for the material contained within the vial of "PD-L1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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