Human TIMP-1 CLIA Kit | TIMP-1 clia kit

TIMP-1 (Human) LumiAb ELISA Kit

Cat. Num. AAA318561

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• Reactivity: Human
• Synonyms: TIMP-1; N/A; TIMP-1 (Human) LumiAb ELISA Kit; Human TIMP-1; Metalloproteinase inhibitor 1; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; EPA; CLGI; TIMP; TIMP-1 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Thrombospondin-1 ELISA is capable of recognizing both recombinant and naturally produced Human Thrombospondin-1 proteins. The antigens listed below were tested at 1000 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 207
• Assay Type: Quantitative Sandwich
• Sensitivity: 400 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for TIMP-1 clia kit

Principle of the Assay: The LumiAb Human Thrombospondin-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Thrombospondin-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Thrombospondin-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Thrombospondin-1 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. The protein binds heparin and may play a role in dentinogenesis and/or the maintenance of dentin and dental pulp. Thrombospondin-1 is a ligand for CD36-mediating anti-angiogenic properties. It is a homotrimer and disulfide-linked. Thrombospondin-1 interacts (via the TSP type I repeats) with HRG; the interaction blocks the anti-angiogenic effect of THBS1 with CD36. Thrombospondin-1 can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3.

Product Categories/Family for TIMP-1 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4507509
• NCBI GeneID: 7076
• NCBI Accession #: NP_003245.1
• NCBI GenBank Nucleotide #: NM_003254.3
• UniProt Accession #: P01033
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• NCBI Protein Information: metalloproteinase inhibitor 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN