Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit | MPST elisa kit
Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit
Gene Names
MPST; MST; TST2; TUM1
Reactivity
Human
Synonyms
3-Mercaptopyruvate Sulfurtransferase (MPST); N/A; Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit; MPST; MGC24539; MST; TST2; 3-mercaptopyruvate sulfurtransferase; OTTHUMP00000197788; OTTHUMP00000197791; human liver rhodanese; MPST elisa kit
Reactivity
Human
Specificity
Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPST. No significant cross-reactivity or interference between Human MPST and analogues was observed.
Sequence Length
297
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
30 pg/ml - 480 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MPST elisa kit
Background: 3-Mercaptopyruvate sulfurtransferase (MPST) catalyzes the desulfuration of 3-mercaptopyruvate to generate an enzyme-bound hydropersulfide. Subsequently, MPST transfers the persulfide's outer sulfur atom to proteins or small molecule acceptors. MPST activity is known to be involved in hydrogen sulfide generation, tRNA thiolation, protein urmylation and cyanide detoxification. Tissue-specific changes in MPST expression correlate with ageing and the development of metabolic disease. Deletion and overexpression experiments suggest that MPST contributes to oxidative stress resistance, mitochondrial respiratory function and the regulation of fatty acid metabolism.
Principle of the Assay: Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPST present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MPST detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPST present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MPST detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
3-mercaptopyruvate sulfurtransferase isoform 2
NCBI Official Synonym Full Names
mercaptopyruvate sulfurtransferase
NCBI Official Symbol
MPST
NCBI Official Synonym Symbols
MST; TST2; TUM1
NCBI Protein Information
3-mercaptopyruvate sulfurtransferase
UniProt Protein Name
3-mercaptopyruvate sulfurtransferase
UniProt Gene Name
MPST
UniProt Synonym Gene Names
TST2; MST
UniProt Entry Name
THTM_HUMAN
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Product Notes
The Human MPST mpst (Catalog #AAA331709) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331709 ELISA Kit recognizes Human MPST. It is sometimes possible for the material contained within the vial of "3-Mercaptopyruvate Sulfurtransferase (MPST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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