Human Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain (ACADVL) ELISA Kit | ACADVL elisa kit

Human Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain (ACADVL) ELISA Kit

Cat. Num. AAA189855

• Gene Names: ACADVL; ACAD6; LCACD; VLCAD
• Reactivity: Human
• Synonyms: Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain (ACADVL); N/A; Human Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain (ACADVL) ELISA Kit; VLCAD; LCACD; ACAD6; Very long-chain specific acyl-CoA dehydrogenase; mitochondrial; ACADVL/Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain; ACADVL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACADVL. No significant cross-reactivity or interference between ACADVL and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACADVL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACADVL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life); Shelf Life: 8 months; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for ACADVL elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ACADVL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ACADVL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACADVL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACADVL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACADVL in human tissue homogenates, cell lysates and other biological fluids.

Product Categories/Family for ACADVL elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4557235
• NCBI GeneID: 37
• NCBI Accession #: NP_000009.1
• NCBI GenBank Nucleotide #: NM_000018.3
• UniProt Accession #: P49748; O76056; Q8WUL0; B4DEB6; F5H2A9
• Molecular Weight: 72,927 Da
• NCBI Official Full Name: very long-chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase, very long chain
• NCBI Official Symbol: ACADVL
• NCBI Official Synonym Symbols: ACAD6; LCACD; VLCAD
• NCBI Protein Information: very long-chain specific acyl-CoA dehydrogenase, mitochondrial; acyl-Coenzyme A dehydrogenase, very long chain
• UniProt Protein Name: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Gene Name: ACADVL
• UniProt Synonym Gene Names: VLCAD; VLCAD
• UniProt Entry Name: ACADV_HUMAN