Human Adenosine Deaminase (ADA) ELISA Kit | ADA elisa kit
Human Adenosine Deaminase (ADA) ELISA Kit
Reactivity
Human
Synonyms
Adenosine Deaminase (ADA); N/A; Human Adenosine Deaminase (ADA) ELISA Kit; Adenosine Aminohydrolase; ADA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ADA. No significant cross-reactivity or interference between ADA and analogues was observed.
Sequence Length
363
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.061ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for ADA elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADA in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ADA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ADA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ADA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ADA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ADA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,764 Da
NCBI Official Full Name
adenosine deaminase
NCBI Official Synonym Full Names
adenosine deaminase
NCBI Official Symbol
ADA
NCBI Protein Information
adenosine deaminase
UniProt Protein Name
Adenosine deaminase
UniProt Gene Name
ADA
UniProt Synonym Gene Names
ADA1
UniProt Entry Name
ADA_HUMAN
