Standard Curve (Sample)
(Fig.1. Human Adenosine deaminase,ADA Standard Curve.)
Human Adenosine Deaminase, ADA ELISA Kit | ADA elisa kit
Human Adenosine Deaminase, ADA ELISA Kit
Reactivity
Human
Synonyms
Adenosine Deaminase, ADA; N/A; Human Adenosine Deaminase, ADA ELISA Kit; ADA; Adenosine aminohydrolase; ADA elisa kit
Reactivity
Human
Specificity
Human Adenosine deaminase,ADA ELISA Kit has high sensitivity and excellent specificity for detection of Human ADA. No significant cross-reactivity or interference between Human ADA and analogues was observed.
Sequence Length
363
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Adenosine deaminase,ADA Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Adenosine deaminase,ADA Standard Curve.)
Related Product Information for ADA elisa kit
Description: This Human Adenosine deaminase,ADA ELISA Kit employs a two-site sandwich ELISA to quantitate ADA in samples. An antibody specific for ADA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Adenosine deaminase is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues.Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. ADA exists in both small form (as a monomer) and large form (as a dimer-complex). In the monomer form, the enzyme is a polypeptide chain, folded into eight strands of parallel alpha/beta barrels, which surround a central deep pocket that is the active site. In addition to the eight central beta-barrels and eight peripheral alpha-helices, ADA also contains five additional helices: residues 19-76 fold into three helices, located between beta1 and alpha1 folds; and two antiparallel carboxy-terminal helices are located across the amino-terminal of the beta-barrel. Its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.
Background: Adenosine deaminase is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues.Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. ADA exists in both small form (as a monomer) and large form (as a dimer-complex). In the monomer form, the enzyme is a polypeptide chain, folded into eight strands of parallel alpha/beta barrels, which surround a central deep pocket that is the active site. In addition to the eight central beta-barrels and eight peripheral alpha-helices, ADA also contains five additional helices: residues 19-76 fold into three helices, located between beta1 and alpha1 folds; and two antiparallel carboxy-terminal helices are located across the amino-terminal of the beta-barrel. Its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adenosine deaminase isoform 1
NCBI Official Synonym Full Names
adenosine deaminase
NCBI Official Symbol
ADA
NCBI Protein Information
adenosine deaminase
UniProt Protein Name
Adenosine deaminase
UniProt Gene Name
ADA
UniProt Synonym Gene Names
ADA1
UniProt Entry Name
ADA_HUMAN
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Product Notes
The Human ADA ada (Catalog #AAA331098) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331098 ELISA Kit recognizes Human ADA. It is sometimes possible for the material contained within the vial of "Adenosine Deaminase, ADA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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