Standard Curve (Sample)
(Fig.1. Human Adenosine receptor A2a (ADORA2A) Standard Curve.)
Human Adenosine Receptor A2a (ADORA2A) ELISA Kit | ADORA2A elisa kit
Human Adenosine Receptor A2a (ADORA2A) ELISA Kit
Gene Names
ADORA2A; A2aR; RDC8; ADORA2
Reactivity
Human
Synonyms
Adenosine Receptor A2a (ADORA2A); N/A; Human Adenosine Receptor A2a (ADORA2A) ELISA Kit; ADORA2A; ADORA2; RDC8; hA2aR; adenosine A2 receptor; adenosine receptor subtype A2a; ADORA2A elisa kit
Reactivity
Human
Specificity
Human Adenosine receptor A2a (ADORA2A) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADORA2A. No significant cross-reactivity or interference between Human ADORA2A and analogues was observed.
Sequence Length
412
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Adenosine receptor A2a (ADORA2A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Adenosine receptor A2a (ADORA2A) Standard Curve.)
Related Product Information for ADORA2A elisa kit
Description: This Human Adenosine receptor A2a (ADORA2A) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA2A in samples. An antibody specific for ADORA2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADORA2a is a member of the G protein-coupled receptor (GPCR) family which possess seven transmembrane alpha helices. The crystallographic structure of the adenosine A2A receptor PDB 3EML (see figure to the right) reveals a ligand binding pocket distinct from that of other structurally determined GPCRs. Adenosine A2a Receptoris one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein-coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.As with the A1, the A2A receptors are believed to play a role in regulating myocardial oxygen consumption and coronary blood flow.
Background: ADORA2a is a member of the G protein-coupled receptor (GPCR) family which possess seven transmembrane alpha helices. The crystallographic structure of the adenosine A2A receptor PDB 3EML (see figure to the right) reveals a ligand binding pocket distinct from that of other structurally determined GPCRs. Adenosine A2a Receptoris one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein-coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.As with the A1, the A2A receptors are believed to play a role in regulating myocardial oxygen consumption and coronary blood flow.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adenosine receptor A2a
NCBI Official Synonym Full Names
adenosine A2a receptor
NCBI Official Symbol
ADORA2A
NCBI Official Synonym Symbols
A2aR; RDC8; ADORA2
NCBI Protein Information
adenosine receptor A2a
UniProt Protein Name
Adenosine receptor A2a
UniProt Gene Name
ADORA2A
UniProt Synonym Gene Names
ADORA2
UniProt Entry Name
AA2AR_HUMAN
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Product Notes
The Human ADORA2A adora2a (Catalog #AAA331130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331130 ELISA Kit recognizes Human ADORA2A. It is sometimes possible for the material contained within the vial of "Adenosine Receptor A2a (ADORA2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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