Application Data
(This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of Leptin at 20ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.)
Human AFP/alpha-fetoprotein ELISA Kit | AFP elisa kit
Human AFP/alpha-fetoprotein ELISA Kit
Reactivity
Human
Synonyms
AFP/alpha-fetoprotein; N/A; Human AFP/alpha-fetoprotein ELISA Kit; Alpha-fetoprotein ELISA Kit, Human; alpha-fetoprotein; AFPD ELISA Kit, Human; FETA ELISA Kit, Human; HPAFP ELISA Kit, Human; AFP elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human AFP/alpha-fetoprotein concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
15.63-1000pg/mL
Sensitivity
5.59pg/mL
Conjugation
HRP
Interference
Preparations of Leptin at 20ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of Leptin at 20ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.)
Application Data
(This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of Leptin at 20ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for AFP elisa kit
This Alpha-fetoprotein ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Alpha-fetoprotein protein in Serum. It contains recombinant Human Alpha-fetoprotein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
Background: Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
References
Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function:
Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid:
comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid:
comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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Product Notes
The Human AFP (Catalog #AAA258447) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258447 ELISA Kit recognizes Human AFP. It is sometimes possible for the material contained within the vial of "AFP/alpha-fetoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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