Standard Curve (Sample)
Human Aggrecan PicoKine ELISA Kit | ACAN elisa kit
Human Aggrecan PicoKine ELISA Kit
Gene Names
ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
Reactivity
Human
Synonyms
Aggrecan PicoKine; N/A; Human Aggrecan PicoKine ELISA Kit; Aggrecan core protein; ACAN; AGC 1; AGC1; AGCAN; Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan 1; Aggrecan proteoglycan; Aggrecan structural proteoglycan of cartilage; Aggrecan1; ATEGQV; Cartilage specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122; Chondroitin sulfate proteoglycan core protein 1; CSPG 1; CSPG1; CSPGCP; JSCATE; Large aggregating proteoglycan; mcspg; mgsk16; MSK 16; MSK16; SEDK ; aggrecan; ACAN elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human ACAN
Sequence Length
2415
Samples
Cell culture supernatants, serum and urine
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<50 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ACAN elisa kit
Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
Principle of the Assay: The Picokine™ Human ACAN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ACAN with a 96-well strip plate that is pre-coated with antibody specific for ACAN. The detection antibody is a biotinylated antibody specific for ACAN. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ACAN with immunogen: Expression system for standard: NS0; Immunogen sequence: V20-R675. The kit is analytically validated with ready-to-use reagents. To measure Human ACAN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human ACAN in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ACAN in the sample.
Principle of the Assay: The Picokine™ Human ACAN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ACAN with a 96-well strip plate that is pre-coated with antibody specific for ACAN. The detection antibody is a biotinylated antibody specific for ACAN. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ACAN with immunogen: Expression system for standard: NS0; Immunogen sequence: V20-R675. The kit is analytically validated with ready-to-use reagents. To measure Human ACAN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human ACAN in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ACAN in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
239,235 Da
NCBI Official Full Name
Aggrecan core protein
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
ACAN
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
NCBI Protein Information
aggrecan core protein
UniProt Protein Name
Aggrecan core protein
UniProt Gene Name
ACAN
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1
UniProt Entry Name
PGCA_HUMAN
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Product Notes
The Human ACAN acan (Catalog #AAA46140) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46140 ELISA Kit recognizes Human ACAN. It is sometimes possible for the material contained within the vial of "Aggrecan PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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