Standard Curve (Sample)
Human AKR1C3 ELISA Kit | AKR1C3 elisa kit
Human AKR1C3 PicoKine ELISA Kit
Gene Names
AKR1C3; DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
Reactivity
Human
Synonyms
AKR1C3; N/A; Human AKR1C3 PicoKine ELISA Kit; Human AKR1C3; Aldo-keto reductase family 1 member C3 (EC:1.-.-.-); 17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2 (EC:1.1.1.357); 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase (EC:1.1.1.112); Prostaglandin F synthase (EC:1.1.1.188); PGFS; Testosterone 17-beta-dehydrogenase 5; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20); DDH1; HSD17B5; KIAA0119; AKR1C3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human AKR1C3
Sequence Length
323
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AKR1C3 elisa kit
Principle of the Assay: The Picokine™ Human AKR1C3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human AKR1C3 with a 96-well strip plate that is pre-coated with antibody specific for AKR1C3. The detection antibody is a biotinylated antibody specific for AKR1C3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human AKR1C3 with immunogen: Expression system for standard: E.coli, Immunogen sequence: M1-Y323. The kit is analytically validated with ready to use reagents. To measure Human AKR1C3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human AKR1C3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human AKR1C3 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Aldo-keto reductase family 1 member C3
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member C3
NCBI Official Symbol
AKR1C3
NCBI Official Synonym Symbols
DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
NCBI Protein Information
aldo-keto reductase family 1 member C3
UniProt Protein Name
Aldo-keto reductase family 1 member C3
UniProt Gene Name
AKR1C3
UniProt Synonym Gene Names
DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS
UniProt Entry Name
AK1C3_HUMAN
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Product Notes
The Human AKR1C3 akr1c3 (Catalog #AAA125206) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125206 ELISA Kit recognizes Human AKR1C3. It is sometimes possible for the material contained within the vial of "AKR1C3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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