Human ALCAM ELISA Kit | ALCAM elisa kit

Human ALCAM PicoKine ELISA Kit

Cat. Num. AAA45912

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Human
• Synonyms: ALCAM; N/A; Human ALCAM PicoKine ELISA Kit; CD166 antigen; activated leukocyte cell adhesion molecule; Activated leukocyte cell adhesion molecule; ALCAM protein; CD 166; CD166; CD166_HUMAN; FLJ3851; FLJ38514; MEMD; MGC71733; ALCAM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human ALCAM
• Sequence Length: 583
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human ALCAM PicoKine ELISA Kit standard curve)

Related Product Information for ALCAM elisa kit

Principle of the assay: human ALCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ALCAM has been precoated onto 96-well plates. Standards(NSO, W28-A526) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for ALCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ALCAM amount of sample captured in plate.
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

NCBI and Uniprot Product Information

• NCBI GI #: 118572629
• NCBI GeneID: 214
• UniProt Accession #: Q13740; O60892; Q1HGM8; Q1HGM9; Q6PEY4; Q6ZS95; B2RNS3; B4DTU0
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN