Standard Curve (Sample)
Human Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit | MGAT4A elisa kit
Human Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit
Gene Names
MGAT4A; GNT-IV; GnT-4a; GNT-IVA
Reactivity
Human
Synonyms
Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A); N/A; Human Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit; Human Alpha 1, 3 mannosyl glycoprotein 4 b N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit; MGAT4A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MGAT4A. No significant cross-reactivity or interference between MGAT4A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MGAT4A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MGAT4A elisa kit
Intended Uses: This MGAT4A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MGAT4A. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: MGAT4A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MGAT4A antibody and an MGAT4A-HRP conjugate. The assay sample and buffer are incubated together with MGAT4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGAT4A concentration since MGAT4A from samples and MGAT4A-HRP conjugate compete for the anti-MGAT4A antibody binding site. Since the number of sites is limited, as more sites are occupied by MGAT4A from the sample, fewer sites are left to bind MGAT4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGAT4A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MGAT4A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MGAT4A antibody and an MGAT4A-HRP conjugate. The assay sample and buffer are incubated together with MGAT4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGAT4A concentration since MGAT4A from samples and MGAT4A-HRP conjugate compete for the anti-MGAT4A antibody binding site. Since the number of sites is limited, as more sites are occupied by MGAT4A from the sample, fewer sites are left to bind MGAT4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGAT4A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MGAT4A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,946 Da
NCBI Official Full Name
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform 1
NCBI Official Synonym Full Names
mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
NCBI Official Symbol
MGAT4A
NCBI Official Synonym Symbols
GNT-IV; GnT-4a; GNT-IVA
NCBI Protein Information
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A; glcNAc-T IVa; N-acetylglucosaminyltransferase IVa; UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV; alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa; UDP-N-acetylglucosamine:alpha1,3-d-mannoside beta1,4-N-acetylglucosaminyltransferase; mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme A; UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
UniProt Protein Name
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
UniProt Gene Name
MGAT4A
UniProt Synonym Gene Names
GlcNAc-T IVa; GnT-IVa; N-acetylglucosaminyltransferase IVa
UniProt Entry Name
MGT4A_HUMAN
Similar Products
Product Notes
The Human MGAT4A mgat4a (Catalog #AAA244912) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244912 ELISA Kit recognizes Human MGAT4A. It is sometimes possible for the material contained within the vial of "Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.