Standard Curve (Sample)
(Fig.1. Human Alpha-1-antitrypsin (SERPINA1) Standard Curve.)
Human Alpha-1-Antitrypsin (SERPINA1) ELISA Kit | SERPINA1 elisa kit
Human Alpha-1-Antitrypsin (SERPINA1) ELISA Kit
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
Reactivity
Human
Synonyms
Alpha-1-Antitrypsin (SERPINA1); N/A; Human Alpha-1-Antitrypsin (SERPINA1) ELISA Kit; SERPINA1; A1A; A1-AT; AAT; MGC23330; MGC9222; PI; PI1; PRO2275; OTTHUMP00000197150; protease inhibitor 1 (anti-elastase); alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor; clade A; member 1; serine; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; SERPINA1 elisa kit
Reactivity
Human
Specificity
Human Alpha-1-antitrypsin (SERPINA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA1. No significant cross-reactivity or interference between Human SERPINA1 and analogues was observed.
Sequence Length
418
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Alpha-1-antitrypsin (SERPINA1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Alpha-1-antitrypsin (SERPINA1) Standard Curve.)
Related Product Information for SERPINA1 elisa kit
Description: This Human Alpha-1-antitrypsin (SERPINA1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA1 in samples. An antibody specific for SERPINA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: alpha1-antitrypsin, an acute phase protein, is the prototypic member of the serpin super family and a major inhibitor of serine proteases. As an acute phase protein, alpha 1-antitrypsin is thought to play an important role in limiting host tissue injury at sites of inflammation. A1AT is a 52-kDa serpin (serine protease inhibitor), and, in medicine, it is considered the most prominent serpin; the terms alpha 1-antitrypsin and protease inhibitor (Pi) are often used interchangeably.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).
Background: alpha1-antitrypsin, an acute phase protein, is the prototypic member of the serpin super family and a major inhibitor of serine proteases. As an acute phase protein, alpha 1-antitrypsin is thought to play an important role in limiting host tissue injury at sites of inflammation. A1AT is a 52-kDa serpin (serine protease inhibitor), and, in medicine, it is considered the most prominent serpin; the terms alpha 1-antitrypsin and protease inhibitor (Pi) are often used interchangeably.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin family A member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
UniProt Entry Name
A1AT_HUMAN
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Product Notes
The Human SERPINA1 serpina1 (Catalog #AAA330915) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330915 ELISA Kit recognizes Human SERPINA1. It is sometimes possible for the material contained within the vial of "Alpha-1-Antitrypsin (SERPINA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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