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IP (Immunoprecipitation)
(Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)
Mouse SERPINA1 / Alpha 1 Antitrypsin Monoclonal Antibody | anti-SERPINA1 antibody
Mouse Monoclonal [clone 11G2] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Dog, Human, Monkey
Applications
Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Protein A/G Purified
Synonyms
SERPINA1 / Alpha 1 Antitrypsin, Antibody; Mouse Monoclonal [clone 11G2] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin; Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 11G2) IHC-plus; SERPINA1; A1A; A1AT; AAT; Alpha 1 Antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha1AT; PRO2275; Alpha-1-antitrypsin null; PI1; Serpin A1; Human SERPINA1; anti-SERPINA1 antibody
Host
Mouse
Reactivity
Dog, Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11G2
Specificity
Human SERPINA1 / Alpha 1 Antitrypsin
Purity/Purification
Protein A/G Purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
418
Applicable Applications for anti-SERPINA1 antibody
IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Protein expressed in 293T cell transfected with human SERPINA1 expression vector.
Immunogen Type
Transfected Cells
Immunogen
SERPINA1 / Alpha 1 Antitrypsin antibody was raised against protein expressed in 293T cell transfected with human SERPINA1 expression vector.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
IP (Immunoprecipitation)
(Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.)
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.)
WB (Western Blot)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.)
WB (Western Blot)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.)
IHC (Immunohistochemistry)
(Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,755 Da
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin; serpin A1; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
UniProt Entry Name
A1AT_HUMAN
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Product Notes
The SERPINA1 serpina1 (Catalog #AAA52557) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 11G2] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin reacts with Dog, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINA1 / Alpha 1 Antitrypsin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SERPINA1 serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINA1 / Alpha 1 Antitrypsin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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