Standard Curve (Sample)
Human Alpha Fetoprotein (AFP) ELISA Kit | AFP elisa kit
Human Alpha Fetoprotein (AFP) ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Alpha Fetoprotein (AFP); N/A; Human Alpha Fetoprotein (AFP) ELISA Kit; Alpha Fetoprotein; ELISA Kit; AFP; AFP elisa kit
Reactivity
Human
Specificity
Human Alpha Fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human Alpha Fetoprotein (AFP), No significant cross-reactivity or interference between Human Alpha Fetoprotein (AFP) and analogues was observed.
Assay Type
Sandwich
Samples
Serum, Plasma, Cell culture supernatants
Detection Range
0.625-40 ng/mL
Sensitivity
0.313 ng/mL
Applicable Applications for AFP elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?10%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?12%
Preparation and Storage
Store at 4 degree C for 12 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AFP elisa kit
Principle of the Assay: Alpha-fetoprotein (AFP) is a highly specific and sensitive tumor marker for primary liver cancer. Its Cutoff value is generally 20 ug/L, when more than 500 ug/L or content rising, more certain significance.Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.
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Product Notes
The Human AFP (Catalog #AAA332604) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332604 ELISA Kit recognizes Human AFP. AAA Biotech's Alpha Fetoprotein (AFP) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the AFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Fetoprotein (AFP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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