Standard Curve (Sample)
Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit | FTO elisa kit
Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit
Gene Names
FTO; GDFD; ALKBH9; BMIQ14
Reactivity
Human
Synonyms
Alpha-ketoglutarate-dependent dioxygenase FTO; N/A; Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit; Fat mass and obesity-associated protein; FTO; KIAA1752; 1.14.11.-; FTO elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human Alpha-ketoglutarate-dependent dioxygenase FTO. No significant cross-reactivity or interference was observed.
Sequence Length
505
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.31 ng/mL -20.0 ng/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FTO elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Alpha-ketoglutarate-dependent dioxygenase FTO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutarate-dependent dioxygenase FTO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-ketoglutarate-dependent dioxygenase FTO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-ketoglutarate-dependent dioxygenase FTO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-ketoglutarate-dependent dioxygenase FTO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutarate-dependent dioxygenase FTO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-ketoglutarate-dependent dioxygenase FTO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-ketoglutarate-dependent dioxygenase FTO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-ketoglutarate-dependent dioxygenase FTO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,218 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO
NCBI Official Synonym Full Names
fat mass and obesity associated
NCBI Official Symbol
FTO
NCBI Official Synonym Symbols
GDFD; ALKBH9; BMIQ14
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Gene Name
FTO
UniProt Synonym Gene Names
KIAA1752
UniProt Entry Name
FTO_HUMAN
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Product Notes
The Human FTO fto (Catalog #AAA196214) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA196214 ELISA Kit recognizes Human FTO. It is sometimes possible for the material contained within the vial of "Alpha-ketoglutarate-dependent dioxygenase FTO, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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