Human ANGPTL4/Angiopoietin Like 4/ARP4 ELISA Kit | ANGPTL4 elisa kit

Human ANGPTL4/Angiopoietin Like 4/ARP4 Quick ELISA Kit

Cat. Num. AAA125886

• Gene Names: ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• Reactivity: Human
• Synonyms: ANGPTL4/Angiopoietin Like 4/ARP4; N/A; Human ANGPTL4/Angiopoietin Like 4/ARP4 Quick ELISA Kit; Angiopoietin-like Protein 4/ANGPTL4|Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein; ANGPTL4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ANGPTL4
• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 625pg/ml-40,000pg/ml
• Sensitivity: <20pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|625|1250|2500|5000|10000|20000|40000; O.D.|0.013|0.159|0.252|0.395|0.620|0.991|1.502|2.064
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|1210|4752|21031|; Standard deviation|81.07|261.36|967.43|; CV(%)|6.7%|5.5%|4.6%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|1116|4712|21659; Standard deviation|81.47|325.13|974.66; CV(%)|7.3%|6.9%|4.5%

• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 1210|1128|1302|1147|1197|74.2|6.2%; Sample 2: 4752|4862|5145|4510|4817|269.77|5.6%; Sample 3: 21031|21450|20486|18843|20453|859.01|4.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L165-S406
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human ANGPTL4/Angiopoietin Like 4/ARP4 with <20pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human ANGPTL4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human ANGPTL4 with immunogen: Expression system for standard: NS0; Immunogen sequence: L165-S406.; ; To measure Human ANGPTL4, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ANGPTL4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANGPTL4 in the sample.
• Assay Procedure Step 1: 40000pg/ml, 20000pg/ml,10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Application Data

Related Product Information for ANGPTL4 elisa kit

Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13. 3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Product Categories/Family for ANGPTL4 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 25008123
• NCBI GeneID: 51129
• UniProt Accession #: Q9BY76; Q53HQ6; Q53HU1; Q6UXN0; Q9HBV4; Q9NZU4; Q9Y5B3; A8MY84; B4E089; D6W670; F5H0I2
• Molecular Weight: 406
• NCBI Official Full Name: Angiopoietin-related protein 4
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: ANGPTL4
• NCBI Official Synonym Symbols: NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• NCBI Protein Information: angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angi
• UniProt Protein Name: Angiopoietin-related protein 4
• UniProt Gene Name: ANGPTL4
• UniProt Synonym Gene Names: ARP4; HFARP; PGAR; HFARP
• UniProt Entry Name: ANGL4_HUMAN