Standard Curve (Sample)
Human ANGPTL4 ELISA Kit | ANGPTL4 elisa kit
Human ANGPTL4 PicoKine ELISA Kit
Gene Names
ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
Reactivity
Human
Synonyms
ANGPTL4; N/A; Human ANGPTL4 PicoKine ELISA Kit; Angiopoietin-related protein 4; angiopoietin-like 4; 425O18 1; Angiopoietin like 4; Angiopoietin related protein 4; ANGPT L2 ; ANGPT L4; ANGPTL2; ANGPTL4 ; Fasting induced adipose factor ; FIAF; Fibrinogen/angiopoietin related protein; Hepatic fibrinogen/angiopoietin related protein ; HFARP; Major histocompatibility complex region NG27; NL2; Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein ; PGAR; pp1158 ; PPARG angiopoietin related protein ; PSEC0166 ; Secreted protein Bk89; Weakly similar to angiopoietin 1; ANGPTL4 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human ANGPTL4
Sequence Length
406
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
625 pg/ml - 40,000 pg/ml
Sensitivity
<20 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ANGPTL4 elisa kit
Background: Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
Principle of the Assay: The Picokine™ Human ANGPTL4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ANGPTL4 with a 96-well strip plate that is pre-coated with antibody specific for ANGPTL4. The detection antibody is a biotinylated antibody specific for ANGPTL4. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ANGPTL4 with immunogen: Expression system for standard: NS0; Immunogen sequence: L165-S406. The kit is analytically validated with ready-to-use reagents. To measure Human ANGPTL4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human ANGPTL4 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANGPTL4 in the sample.
Principle of the Assay: The Picokine™ Human ANGPTL4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ANGPTL4 with a 96-well strip plate that is pre-coated with antibody specific for ANGPTL4. The detection antibody is a biotinylated antibody specific for ANGPTL4. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ANGPTL4 with immunogen: Expression system for standard: NS0; Immunogen sequence: L165-S406. The kit is analytically validated with ready-to-use reagents. To measure Human ANGPTL4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human ANGPTL4 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANGPTL4 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26,853 Da
NCBI Official Full Name
Angiopoietin-related protein 4
NCBI Official Synonym Full Names
angiopoietin-like 4
NCBI Official Symbol
ANGPTL4
NCBI Official Synonym Symbols
NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
NCBI Protein Information
angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
UniProt Protein Name
Angiopoietin-related protein 4
UniProt Gene Name
ANGPTL4
UniProt Synonym Gene Names
ARP4; HFARP; PGAR; HFARP
UniProt Entry Name
ANGL4_HUMAN
Similar Products
Product Notes
The Human ANGPTL4 angptl4 (Catalog #AAA45851) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45851 ELISA Kit recognizes Human ANGPTL4. It is sometimes possible for the material contained within the vial of "ANGPTL4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.