Standard Curve (Sample)
Human Anti-150 kDa Oxygen Regulated Protein Antibody (Anti-ORP150) ELISA Kit | ORP150 elisa kit
Human Anti-150 kDa Oxygen Regulated Protein Antibody (Anti-ORP150) ELISA Kit
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human
Synonyms
Anti-150 kDa Oxygen Regulated Protein Antibody (Anti-ORP150); N/A; Human Anti-150 kDa Oxygen Regulated Protein Antibody (Anti-ORP150) ELISA Kit; HYOU1; HSP12A; Grp170; Glucose-Regulated Protein 170; Hypoxia Up Regulated 1; ORP150/150 kDa Oxygen Regulated Protein; ORP150 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-ORP150. No significant cross-reactivity or interference between Anti-ORP150 and analogues was observed.
Samples
Human serum, plasma and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.14ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-ORP150 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-ORP150 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ORP150 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with ORP150. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-ORP150 will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-ORP150 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-ORP150 in human serum, plasma and other biological fluids.
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-ORP150 in human serum, plasma and other biological fluids.
Product Categories/Family for ORP150 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
111,335 Da
NCBI Official Full Name
HYOU1 protein
NCBI Official Synonym Full Names
hypoxia up-regulated 1
NCBI Official Symbol
HYOU1
NCBI Official Synonym Symbols
Grp170; HSP12A; ORP150; GRP-170; ORP-150
NCBI Protein Information
hypoxia up-regulated protein 1; glucose-regulated protein 170; 150 kDa oxygen-regulated protein; oxygen regulated protein (150kD); 170 kDa glucose-regulated protein
UniProt Protein Name
Hypoxia up-regulated protein 1
UniProt Gene Name
HYOU1
UniProt Synonym Gene Names
GRP170; ORP150; ORP-150; GRP-170
UniProt Entry Name
HYOU1_HUMAN
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Product Notes
The Human ORP150 hyou1 (Catalog #AAA189625) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189625 ELISA Kit recognizes Human ORP150. It is sometimes possible for the material contained within the vial of "Anti-150 kDa Oxygen Regulated Protein Antibody (Anti-ORP150), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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