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IF (Immunofluorescence)
(Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ORP150 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Rabbit anti-Human ORP150 Monoclonal Antibody | anti-HYOU1 antibody
Recombinant Anti-ORP150 Antibody, Rabbit Monoclonal
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A
Synonyms
ORP150, Antibody; Recombinant Anti-ORP150 Antibody, Rabbit Monoclonal; ORP150/ORP150/HYOU1/HSP12A Antibody, Rabbit MAb; hypoxia up-regulated 1; Anti-GRP-170 Antibody; Anti-Grp170 Antibody; Anti-HSP12A Antibody; Anti-ORP-150 Antibody; Anti-ORP150 Antibody; anti-HYOU1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
201
Specificity
Human ORP150/HYOU1/HSP12A.
No cross-reactivity in ELISA with Human Fetuin A
No cross-reactivity in ELISA with Human Fetuin A
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HYOU1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human ORP150/HYOU1/HSP12A protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ORP150/HYOU1/HSP12A (rh ORP150/HYOU1/HSP12A).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ORP150 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ORP150 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
IP (Immunoprecipitation)
(HSP12A was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa)
IP (Immunoprecipitation)
(HSP12A was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa)
WB (Western Blot)
(Anti-HSP12A rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:111 kDaObserved band size:170 kDa)
WB (Western Blot)
(Anti-HSP12A rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:111 kDaObserved band size:170 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)
IHC (Immunohistochemistry)
(Immunochemical staining of human HSP12A in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.)
IHC (Immunohistochemistry)
(Immunochemical staining of human HSP12A in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.)
Related Product Information for anti-HYOU1 antibody
Hypoxia up-regulated protein 1, also known as 15 kDa oxygen-regulated protein, 17 kDa glucose-regulated protein, ORP-15, GRP-17, and HYOU1, is a member of the heat shock protein 7 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP15), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP6), and several isoforms of the two testis-specific HSP7 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in the liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein is up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.
References
Ikeda J., et al., (1997), Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150. Biochem. Biophys. Res. Commun. 230:94-99.Kaneda S., et al., (2000), Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions.J. Biochem. 128:529-538.Takeuchi S., (2006), Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone.Protein J. 25:517-528.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,757 Da
NCBI Official Full Name
hypoxia up-regulated protein 1
NCBI Official Synonym Full Names
hypoxia up-regulated 1
NCBI Official Symbol
HYOU1
NCBI Official Synonym Symbols
Grp170; HSP12A; ORP150; GRP-170; ORP-150
NCBI Protein Information
hypoxia up-regulated protein 1
UniProt Protein Name
Hypoxia up-regulated protein 1
UniProt Gene Name
HYOU1
UniProt Synonym Gene Names
GRP170; ORP150; ORP-150; GRP-170
UniProt Entry Name
HYOU1_HUMAN
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Product Notes
The HYOU1 hyou1 (Catalog #AAA255404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-ORP150 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORP150 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HYOU1 hyou1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORP150, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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