Standard Curve (Sample)
(Fig.1. Human Apoptosis signal regulating kinase 1 (ASK-1) Standard Curve.)
Human Apoptosis Signal Regulating Kinase 1 (ASK-1) ELISA Kit | ASK-1 elisa kit
Human Apoptosis Signal Regulating Kinase 1 (ASK-1) ELISA Kit
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human
Synonyms
Apoptosis Signal Regulating Kinase 1 (ASK-1); N/A; Human Apoptosis Signal Regulating Kinase 1 (ASK-1) ELISA Kit; MAP3K5; RP3-325F22.4; ASK1; MAPKKK5; MEKK5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; ASK-1 elisa kit
Reactivity
Human
Specificity
Human Apoptosis signal regulating kinase 1 (ASK-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP3K5. No significant cross-reactivity or interference between Human MAP3K5 and analogues was observed.
Sequence Length
1374
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Apoptosis signal regulating kinase 1 (ASK-1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Apoptosis signal regulating kinase 1 (ASK-1) Standard Curve.)
Related Product Information for ASK-1 elisa kit
Description: This Human Apoptosis signal regulating kinase 1 (ASK-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP3K5 in samples. An antibody specific for MAP3K5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP3K5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP3K5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP3K5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Apoptosis signal-regulating kinase 1 is an enzyme which in humans is encoded by the MAP3K5 gene. This gene is located on chromosome 6 at locus 6q22.33 and the transcribed protein contains 1,374 amino acids with 11 kinase subdomains. The ASK1 (MAPKKK5) protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; ASK1 does not activate MAPK/ERK.ASK1 found in the inactive form, bound to reduced thioredoxin.When oxidized by a reactive oxygen species, Trx dissociates from ASK1. The ASK1, which is found as a homo-oligodimer, autophosphorylates and becomes an active MAP kinase kinase kinase.
Background: Apoptosis signal-regulating kinase 1 is an enzyme which in humans is encoded by the MAP3K5 gene. This gene is located on chromosome 6 at locus 6q22.33 and the transcribed protein contains 1,374 amino acids with 11 kinase subdomains. The ASK1 (MAPKKK5) protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; ASK1 does not activate MAPK/ERK.ASK1 found in the inactive form, bound to reduced thioredoxin.When oxidized by a reactive oxygen species, Trx dissociates from ASK1. The ASK1, which is found as a homo-oligodimer, autophosphorylates and becomes an active MAP kinase kinase kinase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol
MAP3K5
NCBI Official Synonym Symbols
ASK1; MEKK5; MAPKKK5
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 5
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 5
UniProt Gene Name
MAP3K5
UniProt Synonym Gene Names
ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5
UniProt Entry Name
M3K5_HUMAN
Similar Products
Product Notes
The Human ASK-1 map3k5 (Catalog #AAA331886) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331886 ELISA Kit recognizes Human ASK-1. It is sometimes possible for the material contained within the vial of "Apoptosis Signal Regulating Kinase 1 (ASK-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.