Standard Curve (Sample)
Human BAFF ELISA Kit | BAFF elisa kit
BAFF Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
BAFF; N/A; BAFF Human ELISA Kit; Human BAFF; BAFF elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of. No significant crossreactivity or interference between and analogues was observed.
Assay Type
Sandwich
Samples
Serum, plasma, body fluids, tissue lysates or cell culture supernates
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 2 pg/ml
Applicable Applications for BAFF elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of BAFF in serum, plasma, body fluids, tissue lysates or cell culture supernates.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2-8 degree C for 2 months, or at -20 degree C for 4 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BAFF elisa kit
Background: B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It was expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.As an immunostimulant, BAFF is necessary for maintaining normal immunity.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BAFF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BAFF can be calculated.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BAFF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BAFF can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
BAFF
Similar Products
Product Notes
The Human BAFF (Catalog #AAA13486) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13486 ELISA Kit recognizes Human BAFF. AAA Biotech's BAFF can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of BAFF in serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the BAFF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAFF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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