Mouse anti-Human BAFF Monoclonal Antibody | anti-BAFF antibody

Mouse Monoclonal (IgG1,k) to Human BAFF

Cat. Num. AAA51904

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: BAFF, Antibody; Mouse Monoclonal (IgG1,k) to Human BAFF; Anti-BAFF Antibody IHC-plus; TNFSF13B; B-cell-activating factor; BAFF; ApoL related ligand TALL-1; B-lymphocyte stimulator; CD257; BLYS; Delta4 BAFF; Delta BAFF; TALL-1; TALL1; ZTNF4; THANK; B lymphocyte stimulator; CD257 antigen; TNFSF20; Human BAFF; anti-BAFF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: Human BAFF
• Purity/Purification: Protein G Purified
• Form/Format: Lyophilized from PBS
• Sequence Length: 285

• Applicable Applications for anti-BAFF antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Reconstitution: Reconstitute with Sterile water
• Immunogen Description: E.coli derived recombinant Human BAFF.
• Immunogen Type: Cell extract
• Immunogen: BAFF antibody was raised against e.coli derived recombinant Human BAFF.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8 degree C. Aliquot and freeze at -20 degree C for long term storage. Avoid freeze/thaw cycles.

ELISA

(Sandwich ELISA of BAFF antibody)

WB (Western Blot)

(Western Blot (reducing) of BAFF antibody)

WB (Western Blot)

(Western Blot (non-reducing) of BAFF antibody)

IHC (Immunohistochemistry)

(Anti-BAFF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Related Product Information for anti-BAFF antibody

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kB and c-jun N-terminal Kinase).

Product Categories/Family for anti-BAFF antibody

Family: TNF

NCBI and Uniprot Product Information

• NCBI GI #: 5730097
• NCBI GeneID: 10673
• NCBI Accession #: NP_006564.1
• NCBI GenBank Nucleotide #: NM_006573.4
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN