Standard Curve (Sample)
(Human BCHE PicoKine ELISA Kit standard curve)
Human BCHE ELISA Kit | BCHE elisa kit
Human BCHE PicoKine ELISA Kit
Gene Names
BCHE; E1; CHE1; CHE2
Reactivity
Human
Synonyms
BCHE; N/A; Human BCHE PicoKine ELISA Kit; Acylcholine acylhydrolase; Butyrylcholine esterase; CHE1; CHE2; Choline esterase II; Cholinesterase (serum) 2; Cholinesterase 1; Cholinesterase; E1; Pseudocholinesterase; P06276; butyrylcholinesterase; BCHE elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human BCHE
Sequence Length
602
Samples
Cell culture supernates, tissue homogenates, serum, plasma (heparin, EDTA), saliva, urine and human milk
Assay Type
Quantitative Sandwich
Detection Range
312pg/ml-20000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Human BCHE PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human BCHE PicoKine ELISA Kit standard curve)
Related Product Information for BCHE elisa kit
Principle of the Assay: The Human BCHE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BCHE has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E29-L602) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BCHE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BCHE amount of sample captured in plate.
Background: Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.
Background: Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,418 Da
NCBI Official Full Name
cholinesterase
NCBI Official Synonym Full Names
butyrylcholinesterase
NCBI Official Symbol
BCHE
NCBI Official Synonym Symbols
E1; CHE1; CHE2
NCBI Protein Information
cholinesterase
UniProt Protein Name
Cholinesterase
UniProt Gene Name
BCHE
UniProt Synonym Gene Names
CHE1
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Product Notes
The Human BCHE bche (Catalog #AAA46775) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46775 ELISA Kit recognizes Human BCHE. It is sometimes possible for the material contained within the vial of "BCHE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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