Standard Curve (Sample)
Human bFGF ELISA Kit | bFGF elisa kit
Human bFGF ELISA Kit
Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Human
Applications
ELISA
Synonyms
bFGF; N/A; Human bFGF ELISA Kit; Basic fibroblast growth factor; BFGF; FGF 2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF2; HBGH 2; Heparin binding growth; factor 2; Heparin binding growth factor 2 precursor; OTTMUSP00000046776; Prostatropin; bFGF elisa kit
Reactivity
Human
Specificity
Human bFGF ELISA Kit has high sensitivity and excellent specificity for detection of Human bFGF. No significant cross-reactivity or interference between Human bFGF and analogues was observed
Assay Type
Sandwich
Samples
Serum, Plasma, Cell culture supernatants
Detection Range
15.63 pg/mL-1,000 pg/mL
Sensitivity
8 pg/mL
Applicable Applications for bFGF elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?10%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?12%
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for bFGF elisa kit
Principle of the Assay: FGF basic, also called FGF 2 (fibroblast growth factor 2) or HBGF 2 (heparin binding Growth factor 2), is the most intensively studied of the 22 mitogenic proteins of the FGF family. Family members share 35-60% amino acid (aa) identity, but only FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 18 kDa FGF.Human bFGF ELISA Kit employs a double antibody sandwich method to quantitate Human bFGF in samples. An antibody specific for Human bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Human bFGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450nm wavelength. The bFGF concentration is proportional to the OD450 nm value.
Product Categories/Family for bFGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30,770 Da
NCBI Official Full Name
basic fibroblast growth factor, partial
NCBI Official Synonym Full Names
fibroblast growth factor 2 (basic)
NCBI Official Symbol
FGF2
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
NCBI Protein Information
fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF
UniProt Protein Name
Fibroblast growth factor 2
UniProt Gene Name
FGF2
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2
UniProt Entry Name
FGF2_HUMAN
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Product Notes
The Human bFGF fgf2 (Catalog #AAA332535) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332535 ELISA Kit recognizes Human bFGF. AAA Biotech's bFGF can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the bFGF fgf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "bFGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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