Standard Curve (Sample)
Rat basic fibroblast growth factor, bFGF ELISA Kit | bFGF elisa kit
Rat basic fibroblast growth factor, bFGF ELISA Kit
Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Rat
Synonyms
basic fibroblast growth factor, bFGF; N/A; Rat basic fibroblast growth factor, bFGF ELISA Kit; bFGF elisa kit
Reactivity
Rat
Sequence Length
157
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
2ng/L - 600ng/L
Sensitivity
1.13ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for bFGF elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Basic Fibroblast Growth Factor (also known as bFGF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat bFGF antibody. bFGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat bFGF Antibody is added and binds to bFGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated bFGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat bFGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat bFGF antibody. bFGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat bFGF Antibody is added and binds to bFGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated bFGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat bFGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
22,623 Da
NCBI Official Full Name
basic fibroblast growth factor, partial
NCBI Official Synonym Full Names
fibroblast growth factor 2
NCBI Official Symbol
FGF2
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
NCBI Protein Information
fibroblast growth factor 2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Gene Name
FGF2
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2
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Product Notes
The Rat bFGF fgf2 (Catalog #AAA42442) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA42442 ELISA Kit recognizes Rat bFGF. It is sometimes possible for the material contained within the vial of "basic fibroblast growth factor, bFGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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