Human Bone marrow proteoglycan (PRG2) ELISA Kit | PRG2 elisa kit

Human Bone marrow proteoglycan (PRG2) ELISA Kit

Cat. Num. AAA246200

• Gene Names: PRG2; MBP; BMPG; MBP1
• Reactivity: Human
• Synonyms: Bone marrow proteoglycan (PRG2); N/A; Human Bone marrow proteoglycan (PRG2) ELISA Kit; PRG2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRG2. No significant cross-reactivity or interference between PRG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRG2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Intended Uses: This PRG2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRG2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PRG2 elisa kit

Principle of the Assay: PRG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PRG2 antibody and an PRG2-HRP conjugate. The assay sample and buffer are incubated together with PRG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRG2 concentration since PRG2 from samples and PRG2-HRP conjugate compete for the anti-PRG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRG2 from the sample, fewer sites are left to bind PRG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PRG2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRG2. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for PRG2 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 342837699
• NCBI GeneID: 5553
• NCBI Accession #: NP_001230174.1
• NCBI GenBank Nucleotide #: NM_001243245.1
• Molecular Weight: 25,206 Da
• NCBI Official Full Name: bone marrow proteoglycan isoform 2 preproprotein
• NCBI Official Synonym Full Names: proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• NCBI Official Symbol: PRG2
• NCBI Official Synonym Symbols: MBP; BMPG; MBP1
• NCBI Protein Information: bone marrow proteoglycan; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein
• UniProt Protein Name: Bone marrow proteoglycan
• UniProt Gene Name: PRG2
• UniProt Synonym Gene Names: MBP; BMPG; EMBP; MBP
• UniProt Entry Name: PRG2_HUMAN