Human C-Type Natriuretic Peptide (CNP) ELISA Kit | CNP elisa kit

Human C-Type Natriuretic Peptide (CNP) ELISA Kit

Cat. Num. AAA64107

• Gene Names: NPPC; CNP; CNP2
• Reactivity: Human
• Synonyms: C-Type Natriuretic Peptide (CNP); N/A; Human C-Type Natriuretic Peptide (CNP) ELISA Kit; NPPC; Natriuretic Peptide Precursor C; CNP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CNP. No significant cross-reactivity or interference between CNP and analogues was observed.
• Sequence Length: 126
• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 26.4pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for CNP elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CNP in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13249346
• NCBI GeneID: 4880
• NCBI Accession #: NP_077720.1
• NCBI GenBank Nucleotide #: NM_024409.3
• UniProt Accession #: P23582; Q4ZG41
• Molecular Weight: 13,246 Da
• NCBI Official Full Name: C-type natriuretic peptide preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide C
• NCBI Official Symbol: NPPC
• NCBI Official Synonym Symbols: CNP; CNP2
• NCBI Protein Information: C-type natriuretic peptide
• UniProt Protein Name: C-type natriuretic peptide
• UniProt Gene Name: NPPC
• UniProt Synonym Gene Names: CNP2