Reactivity
Human
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
2.5 ng/mL - 40 ng/mL
Sensitivity
< 0.1 ng/mL
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for C4BPA elisa kit
Background: C4BPA is a Protein Coding gene. This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Diseases associated with C4BPA include Protein S Deficiency and Acute Poststreptococcal Glomerulonephritis. Among its related pathways are Innate Immune System and Complement and coagulation cascades.
Principle of the Assay: Human C4 Binding Protein Alpha (C4BPA) ELISA Kit employs a two-site sandwich ELISA to quantitate C4BPA in samples. An antibody specific for C4BPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4BPA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human C4BPA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of C4BPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human C4 Binding Protein Alpha (C4BPA) ELISA Kit employs a two-site sandwich ELISA to quantitate C4BPA in samples. An antibody specific for C4BPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4BPA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human C4BPA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of C4BPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Product Notes
The Human C4BPA (Catalog #AAA31513) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31513 ELISA Kit recognizes Human C4BPA. It is sometimes possible for the material contained within the vial of "C4 Binding Protein Alpha (C4BPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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