Human Carbonic Anhydrase III, Muscle Specific (CA3) ELISA Kit | CA3 elisa kit

Human Carbonic Anhydrase III, Muscle Specific (CA3) ELISA Kit

Cat. Num. AAA139753

• Gene Names: CA3; Car3; CAIII
• Reactivity: Human
• Synonyms: Carbonic Anhydrase III, Muscle Specific (CA3); N/A; Human Carbonic Anhydrase III, Muscle Specific (CA3) ELISA Kit; CA-III; CAIII; Car3; Carbonate dehydratase III; CA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase III, Muscle Specific (CA3).; No significant cross-reactivity or interference between Carbonic Anhydrase III, Muscle Specific (CA3) and analogues was observed.
• Sequence Length: 260
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: 3.1pg/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase III, Muscle Specific (CA3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase III, Muscle Specific (CA3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for CA3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase III, Muscle Specific (CA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase III, Muscle Specific (CA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase III, Muscle Specific (CA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase III, Muscle Specific (CA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CA3 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4885099
• NCBI GeneID: 761
• NCBI Accession #: NP_005172.1
• NCBI GenBank Nucleotide #: NM_005181.3
• UniProt Accession #: P07451; O60842; B2R867; B3KUC8
• Molecular Weight: 29,557 Da
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase III
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3
• UniProt Protein Name: Carbonic anhydrase 3
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III
• UniProt Entry Name: CAH3_HUMAN