Standard Curve (Sample)
(Human CCL13 PicoKine ELISA Kit standard curve)
Human CCL13/MCP4 ELISA Kit | CCL13 elisa kit
Human CCL13/MCP4 PicoKine Quick ELISA Kit
Gene Names
CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
Reactivity
Human
Applications
ELISA
Synonyms
CCL13/MCP4; N/A; Human CCL13/MCP4 PicoKine Quick ELISA Kit; CCL13 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CCL13
Assay Type
Sandwich
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
7.8 pg/ml - 500 pg/ml
Sensitivity
<10 pg/ml
Applicable Applications for CCL13 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CCL13 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CCL13 PicoKine ELISA Kit standard curve)
Related Product Information for CCL13 elisa kit
Background: Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.
Principle of the Assay: The Quick Picokine™ Human CCL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CCL13 protein as standards. To measure Human CCL13, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CCL13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL13 in the sample.
Principle of the Assay: The Quick Picokine™ Human CCL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CCL13 protein as standards. To measure Human CCL13, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CCL13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL13 in the sample.
Product Categories/Family for CCL13 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
98
NCBI Official Full Name
C-C motif chemokine 13
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 13
NCBI Official Symbol
CCL13
NCBI Official Synonym Symbols
NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
NCBI Protein Information
C-C motif chemokine 13; CK-beta-10; new CC chemokine 1; small-inducible cytokine A13; monocyte chemotactic protein 4; monocyte chemoattractant protein 4; small inducible cytokine subfamily A (Cys-Cys), member 13
UniProt Protein Name
C-C motif chemokine 13
UniProt Gene Name
CCL13
UniProt Synonym Gene Names
MCP4; NCC1; SCYA13; MCP-4
UniProt Entry Name
CCL13_HUMAN
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Product Notes
The Human CCL13 ccl13 (Catalog #AAA126814) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126814 ELISA Kit recognizes Human CCL13. AAA Biotech's CCL13/MCP4 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CCL13 ccl13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL13/MCP4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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