Human CCL18/PARC ELISA Kit | CCL18/PARC elisa kit

Human CCL18/PARC PicoKine Quick ELISA Kit

Cat. Num. AAA128068

• Gene Names: CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• Reactivity: Human
• Applications: ELISA
• Synonyms: CCL18/PARC; N/A; Human CCL18/PARC PicoKine Quick ELISA Kit; CCL18/PARC elisa kit

• Reactivity: Human
• Samples: cell culture supernatants and serum
• Assay Range: 7.8pg/ml-500pg/ml
• Sensitivity: <2pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: A21-A89
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CCL18/PARC elisa kit: ELISA
• Kit Components: Description/Quantity/Volume; Anti-tag Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells; Standard/2, 10 ng/tube; Antibody A/1/3ml; Antibody B/1/3ml; Sample Diluent/1/15ml; TBS-T Wash Buffer (25x)/1/12ml; Color Developing Reagent (TMB)/1/10ml; Stop Solution/1/10ml; Adhesive plate sealers/2/Piece
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Automated plate washer (optional); Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.; Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
• Sample Data:

  Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
  O.D.	0.033	0.100	0.147	0.261	0.446	0.854	1.582	2.058

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	18	49	269	18	52	279
  Standard deviation	0.99	3.52	20.17	1.29	4.62	22.59
  CV (%)	5.5%	7.2%	7.5%	7.2%	8.9%	8.1%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	18	17	18	18	17	0.43	2.5%
  Sample 2	49	56	49	48	50	3.2	6.4%
  Sample 3	269	295	278	296	284	11.45	4%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human CCL18/PARC PicoKine Quick ELISA Kit standard curve)

Related Product Information for CCL18/PARC elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CCL18/PARC elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 4836258
• NCBI GeneID: 6362
• UniProt Accession #: P55774; Q53X71; B5BUM2
• Molecular Weight: 9,849 Da
• NCBI Official Full Name: alternative macrophage activation-associated CC-chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• NCBI Official Symbol: CCL18
• NCBI Official Synonym Symbols: CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• NCBI Protein Information: C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated ch
• UniProt Protein Name: C-C motif chemokine 18
• UniProt Gene Name: CCL18
• UniProt Synonym Gene Names: AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4
• UniProt Entry Name: CCL18_HUMAN