Human CCN1 ELISA Kit | CCN1 elisa kit

Human CCN1 PicoKine Quick ELISA Kit

Cat. Num. AAA128083

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Human
• Applications: ELISA
• Synonyms: CCN1; N/A; Human CCN1 PicoKine Quick ELISA Kit; ; Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1; CYR61; CCN1, GIG1, IGFBP10; CCN1 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk
• Assay Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: CHO; Immunogen sequence: A22-D381
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CCN1 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.037	0.094	0.152	0.258	0.513	0.811	1.520	2.034

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	61	248	814	59	242	806
  Standard deviation	2.86	16.12	55.35	3.3	17.66	64.48
  CV (%)	4.7%	6.5%	6.8%	5.6%	7.3%	8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	61	64	63	58	61	2.29	3.7%
  Sample 2	248	273	263	255	259	9.31	3.5%
  Sample 3	814	907	783	869	843	47.99	5.6%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human CYR61/CCN1 PicoKine ELISA Kit standard curve)

Related Product Information for CCN1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CCN1 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 3023601
• NCBI GeneID: 3491
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7
• Molecular Weight: 381
• NCBI Official Full Name: Protein CYR61
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_HUMAN