Standard Curve (Sample)
Human CD30/TNFRSF8 PicoKine ELISA Kit | TNFRSF8 elisa kit
Human CD30/TNFRSF8 PicoKine ELISA Kit
Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human
Synonyms
CD30/TNFRSF8 PicoKine; N/A; Human CD30/TNFRSF8 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 8; CD 30; CD153; CD30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; tumor necrosis factor receptor superfamily, member 8; TNFRSF8 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFRSF8
Sequence Length
595
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNFRSF8 elisa kit
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
Principle of the Assay: The Picokine™ Human TNFRSF8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF8 with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF8. The detection antibody is a biotinylated antibody specific for TNFRSF8. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human TNFRSF8 protein as standards. To measure Human TNFRSF8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF8 in the sample.
Principle of the Assay: The Picokine™ Human TNFRSF8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF8 with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF8. The detection antibody is a biotinylated antibody specific for TNFRSF8. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human TNFRSF8 protein as standards. To measure Human TNFRSF8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF8 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,935 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 8 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 8
NCBI Official Symbol
TNFRSF8
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
UniProt Gene Name
TNFRSF8
UniProt Synonym Gene Names
CD30; D1S166E
UniProt Entry Name
TNR8_HUMAN
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Product Notes
The Human TNFRSF8 tnfrsf8 (Catalog #AAA46182) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46182 ELISA Kit recognizes Human TNFRSF8. It is sometimes possible for the material contained within the vial of "CD30/TNFRSF8 PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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