Human CD38 ELISA Kit | CD38 elisa kit

CD38 ELISA Kit, Human

Cat. Num. AAA258573

• Reactivity: Human
• Synonyms: CD38; N/A; CD38 ELISA Kit, Human; Human CD38 ELISA Kit; ADPRC 1 ELISA Kit, Human; ADPRC1 ELISA Kit, Human; T10 ELISA Kit, Human; CD38 molecule; CD38 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant Human CD38.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
• Assay Type: Sandwich
• Sensitivity: < 18.45 pg/mL

• Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision: Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
• Preparation and Storage: Unopened Kit: Store at 2-8 degree COpened/Reconstituted Reagents: Please refer to CoA

Standard Curve (Sample)

Related Product Information for CD38 elisa kit

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4⁺, CD8⁺, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD⁺ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

Principle of the Assay: The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human CD38 has been pre-coated onto well plate strips. Standards and samples are added to the wells and Human CD38 present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-Human CD38 antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of Human CD38 bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).

Intended Uses: The kit has been verified by high purity Human CD38 recombinant protein.

Product Categories/Family for CD38 elisa kit

Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.