Standard Curve (Sample)
(Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve)
Human CD40/TNFRSF5 ELISA Kit | CD40 elisa kit
Human CD40/TNFRSF5 PicoKine ELISA Kit
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Synonyms
CD40/TNFRSF5; N/A; Human CD40/TNFRSF5 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 5; CD40 molecule, TNF receptor superfamily member 5; AI326936; B cell associated molecule; B cell associated molecule CD40; B cell surface antigen CD40; Bp50; CD 40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule; CD40 molecule TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; NFRSF5; OTTHUMP00000031699; OTTHUMP00000031700; p50; T BAM; TBAM; TNF receptor superfamily member 5; TNFRSF5; TRAP; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a; CD40 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CD40
Sequence Length
277
Samples
Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve)
Related Product Information for CD40 elisa kit
Principle of the assay: human CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40 has been precoated onto 96-well plates. Standards(NSO, E21-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40 amount of sample captured in plate.
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,259 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 5
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN
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Product Notes
The Human CD40 cd40 (Catalog #AAA45237) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45237 ELISA Kit recognizes Human CD40. It is sometimes possible for the material contained within the vial of "CD40/TNFRSF5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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