Human Cell cycle control protein 50A (TMEM30A) ELISA Kit | TMEM30A elisa kit

Human Cell cycle control protein 50A (TMEM30A) ELISA Kit

Cat. Num. AAA247046

• Gene Names: TMEM30A; CDC50A; C6orf67
• Reactivity: Human
• Synonyms: Cell cycle control protein 50A (TMEM30A); N/A; Human Cell cycle control protein 50A (TMEM30A) ELISA Kit; TMEM30A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TMEM30A. No significant cross-reactivity or interference between TMEM30A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMEM30A and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TMEM30A elisa kit

Principle of the Assay: TMEM30A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TMEM30A antibody and an TMEM30A-HRP conjugate. The assay sample and buffer are incubated together with TMEM30A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM30A concentration since TMEM30A from samples and TMEM30A-HRP conjugate compete for the anti-TMEM30A antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM30A from the sample, fewer sites are left to bind TMEM30A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM30A concentration in each sample is interpolated from this standard curve.

Intended Uses: This TMEM30A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TMEM30A. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for TMEM30A elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 221139888
• NCBI GeneID: 55754
• NCBI Accession #: NP_001137430.1
• NCBI GenBank Nucleotide #: NM_001143958.1
• Molecular Weight: 27,729 Da
• NCBI Official Full Name: cell cycle control protein 50A isoform 2
• NCBI Official Synonym Full Names: transmembrane protein 30A
• NCBI Official Symbol: TMEM30A
• NCBI Official Synonym Symbols: CDC50A; C6orf67
• NCBI Protein Information: cell cycle control protein 50A; P4-ATPase flippase complex beta subunit TMEM30A
• UniProt Protein Name: Cell cycle control protein 50A
• UniProt Gene Name: TMEM30A
• UniProt Synonym Gene Names: C6orf67; CDC50A
• UniProt Entry Name: CC50A_HUMAN