Standard Curve (Sample)
Human Centrosomal protein of 290 kDa (CEP290) ELISA Kit | CEP290 elisa kit
Human Centrosomal protein of 290 kDa (CEP290) ELISA Kit
Gene Names
CEP290; CT87; MKS4; POC3; rd16; BBS14; JBTS5; LCA10; NPHP6; SLSN6; 3H11Ag
Reactivity
Human
Synonyms
Centrosomal protein of 290 kDa (CEP290); N/A; Human Centrosomal protein of 290 kDa (CEP290) ELISA Kit; CEP290 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CEP290. No significant cross-reactivity or interference between CEP290 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CEP290 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CEP290 elisa kit
Principle of the Assay: CEP290 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CEP290 antibody and an CEP290-HRP conjugate. The assay sample and buffer are incubated together with CEP290-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEP290 concentration since CEP290 from samples and CEP290-HRP conjugate compete for the anti-CEP290 antibody binding site. Since the number of sites is limited, as more sites are occupied by CEP290 from the sample, fewer sites are left to bind CEP290-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEP290 concentration in each sample is interpolated from this standard curve.
Intended Uses: This CEP290 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CEP290. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This CEP290 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CEP290. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for CEP290 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180,067 Da
NCBI Official Full Name
centrosomal protein of 290 kDa
NCBI Official Synonym Full Names
centrosomal protein 290kDa
NCBI Official Symbol
CEP290
NCBI Official Synonym Symbols
CT87; MKS4; POC3; rd16; BBS14; JBTS5; LCA10; NPHP6; SLSN6; 3H11Ag
NCBI Protein Information
centrosomal protein of 290 kDa; nephrocytsin-6; tumor antigen se2-2; Meckel syndrome, type 4; CTCL tumor antigen se2-2; cancer/testis antigen 87; prostate cancer antigen T21; POC3 centriolar protein homolog; Bardet-Biedl syndrome 14 protein; monoclonal antibody 3H11 antigen
UniProt Protein Name
Centrosomal protein of 290 kDa
UniProt Gene Name
CEP290
UniProt Synonym Gene Names
BBS14; KIAA0373; NPHP6; Cep290; CT87
UniProt Entry Name
CE290_HUMAN
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Product Notes
The Human CEP290 cep290 (Catalog #AAA27144) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27144 ELISA Kit recognizes Human CEP290. It is sometimes possible for the material contained within the vial of "Centrosomal protein of 290 kDa (CEP290), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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