WB (Western Blot)
(Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody AAA211575 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody BL6289, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).)
Rabbit anti-Human CEP290 Polyclonal Antibody | anti-CEP290 antibody
Rabbit anti-CEP290 Antibody, Affinity Purified
Gene Names
CEP290; CT87; MKS4; POC3; rd16; BBS14; JBTS5; LCA10; NPHP6; SLSN6; 3H11Ag
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CEP290, Antibody; Rabbit anti-CEP290 Antibody, Affinity Purified; Bardet-Biedl syndrome 14 protein; monoclonal antibody 3H11 antigen; Nephrocystin-6; nephrocytsin-6; NPHP6; POC3; POC3 centriolar protein homolog; prostate cancer antigen T21; rd16; SLSN6; MKS4; tumor antigen se2-2; LCA10; JBTS5; CTCL tumor antigen se2-2; CT87; Cep290; centrosomal protein of 290 kDa; centrosomal protein 290kDa; BBS14; 3H11Ag; cancer/testis antigen 87; Meckel syndrome, type 4; Centrosomal protein of 290 kDa; centrosomal protein 290; anti-CEP290 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CEP290 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 2429 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody AAA211575 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody BL6289, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).)
WB (Western Blot)
(Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody AAA211575 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody BL6289, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).)
ICC (Immunocytochemistry)
(Detection of human CEP290 by immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. AAA211575) used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human CEP290 by immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. AAA211575) used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1:100.)
Related Product Information for anti-CEP290 antibody
Centrosomal protein 290 (CEP290) is a member of the nephrocystins, a family of proteins involved in renal cystic diseases. CEP290 and the nephrocystins appear to play a role in cilia and basal body function. Loss-of-function mutations in CEP290 result in Joubert syndrome and related disorders characterized by brain malformations, developmental delay, ataxia, irregular breathing, renal disease, and hepatic fibrosis.
Product Categories/Family for anti-CEP290 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
290,386 Da
NCBI Official Full Name
centrosomal protein of 290 kDa
NCBI Official Synonym Full Names
centrosomal protein 290kDa
NCBI Official Symbol
CEP290
NCBI Official Synonym Symbols
CT87; MKS4; POC3; rd16; BBS14; JBTS5; LCA10; NPHP6; SLSN6; 3H11Ag
NCBI Protein Information
centrosomal protein of 290 kDa; nephrocytsin-6; tumor antigen se2-2; Meckel syndrome, type 4; CTCL tumor antigen se2-2; cancer/testis antigen 87; prostate cancer antigen T21; POC3 centriolar protein homolog; Bardet-Biedl syndrome 14 protein; monoclonal an
UniProt Protein Name
Centrosomal protein of 290 kDa
UniProt Gene Name
CEP290
UniProt Synonym Gene Names
BBS14; KIAA0373; NPHP6; Cep290; CT87
UniProt Entry Name
CE290_HUMAN
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Product Notes
The CEP290 cep290 (Catalog #AAA211575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CEP290 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEP290 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CEP290 cep290 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEP290, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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