Human CFD ELISA Kit | CFD elisa kit

Human CFD PicoKine ELISA Kit

Cat. Num. AAA45753

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Human
• Synonyms: CFD; N/A; Human CFD PicoKine ELISA Kit; Complement factor D; complement factor D (adipsin); Adipsin; ADN; C3 convertase activator; CFAD_HUMAN; Complement factor D preproprotein; D component of complement; DF; PFD; Properdin factor D; CFD elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CFD
• Sequence Length: 253
• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human CFD PicoKine ELISA Kit standard curve)

Related Product Information for CFD elisa kit

Principle of the assay: human CFD ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFD has been precoated onto 96-well plates. Standards(NSO, I26-A253) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for CFD is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CFD amount of sample captured in plate.
Background: Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 158515408
• NCBI GeneID: 1675
• UniProt Accession #: P00746; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4; B4DV76
• Molecular Weight: 27,033 Da
• NCBI Official Full Name: Complement factor D
• NCBI Official Synonym Full Names: complement factor D (adipsin)
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin)
• UniProt Protein Name: Complement factor D
• UniProt Gene Name: CFD
• UniProt Synonym Gene Names: DF; PFD
• UniProt Entry Name: CFAD_HUMAN