Standard Curve (Sample)
(Fig.1. Human Collagen Type I Alpha 1 (COL1A1) Standard Curve.)
Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit | COL1A1 elisa kit
Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit
Reactivity
Human
Synonyms
Collagen Type I Alpha 1 (COL1A1); N/A; Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit; COL1A1; COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain; COL1A1 elisa kit
Reactivity
Human
Specificity
Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human COL1A1. No significant cross-reactivity or interference between Human COL1A1 and analogues was observed.
Sequence Length
1069
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Collagen Type I Alpha 1 (COL1A1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Collagen Type I Alpha 1 (COL1A1) Standard Curve.)
Related Product Information for COL1A1 elisa kit
Description: This Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL1A1 in samples. An antibody specific for COL1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Collagen, type I, alpha 1 is the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage.The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers.
Background: Collagen, type I, alpha 1 is the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage.The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
collagen type I alpha 1, partial
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Product Notes
The Human COL1A1 (Catalog #AAA331985) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331985 ELISA Kit recognizes Human COL1A1. It is sometimes possible for the material contained within the vial of "Collagen Type I Alpha 1 (COL1A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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