Human Collagen Type IV (COL4) ELISA Kit | COL4 elisa kit

Human Collagen Type IV (COL4) ELISA Kit

Cat. Num. AAA183092

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Human
• Synonyms: Collagen Type IV (COL4); N/A; Human Collagen Type IV (COL4) ELISA Kit; Type-IV Collagen; COL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL4. No significant cross-reactivity or interference between COL4 and analogues was observed.
• Sequence Length: 18
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500ng/mL
• Sensitivity: < 3.3ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for COL4 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

References

Mammalian pumilio proteins control cellular morphology, migration, and adhesion; Sci Rep; 2023 Feb 21;13(1):3002.; PMID: 36810759; Erin L Sternburg; Department of Molecular, Cell and Systems Biology, University of California at Riverside, Riverside, CA, 92521, USA.

NCBI and Uniprot Product Information

• NCBI GI #: 4261609
• NCBI GeneID: 1287
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: collagen type IV alpha 5 chain, partial
• NCBI Official Synonym Full Names: collagen type IV alpha 5 chain
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• UniProt Gene Name: COL4A5