Standard Curve (Sample)
Human Contactin-1 ELISA Kit | CNTN1 elisa kit
Human Contactin-1 PicoKine ELISA Kit
Gene Names
CNTN1; F11; contactin-1
Reactivity
Human
Synonyms
Contactin-1; N/A; Human Contactin-1 PicoKine ELISA Kit; Human Contactin-1; CNTN1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CKM
Sequence Length
1010
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
Detection Range
0.15mU/ml-10mU/ml
Sensitivity
<0.05mU/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CNTN1 elisa kit
Principle of the Assay: The Picokine Human CKM Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CKM with a 96-well strip plate that is pre-coated with antibody specific for CKM. The detection antibody is a biotinylated antibody specific for CKM. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CKM with immunogen:. The kit is analytically validated with ready to use reagents. To measure Human CKM, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CKM in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CKM in the sample!!Background/Introduction: Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
contactin-1 preproprotein
NCBI Official Synonym Full Names
contactin 1
NCBI Official Symbol
CNTN1
NCBI Official Synonym Symbols
F11; contactin-1
NCBI Protein Information
contactin-1
Similar Products
Product Notes
The Human CNTN1 (Catalog #AAA125049) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125049 ELISA Kit recognizes Human CNTN1. It is sometimes possible for the material contained within the vial of "Contactin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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