Human Coupling Factor 6 (CF6) ELISA Kit | CF6 elisa kit

Human Coupling Factor 6 (CF6) ELISA Kit

Cat. Num. AAA187939

• Gene Names: ATP5PF; F6; CF6; ATP5; ATPM; ATP5A; ATP5J
• Reactivity: Human
• Synonyms: Coupling Factor 6 (CF6); N/A; Human Coupling Factor 6 (CF6) ELISA Kit; ATP5J; ATP5A; ATP5; ATPM; ATP Synthase,H+ Transporting,Mitochondrial Fo Complex,Subunit F6; ATP synthase-coupling factor 6, mitochondrial; CF6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coupling Factor 6 (CF6).; No significant cross-reactivity or interference between Coupling Factor 6 (CF6) and analogues was observed.
• Sequence Length: 116
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.068ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for CF6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coupling Factor 6 (CF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coupling Factor 6 (CF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coupling Factor 6 (CF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coupling Factor 6 (CF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 183786
• NCBI GeneID: 522
• UniProt Accession #: P18859; J3KQ83
• Molecular Weight: 13,388 Da
• NCBI Official Full Name: coupling factor 6
• NCBI Official Synonym Full Names: ATP synthase peripheral stalk subunit F6
• NCBI Official Symbol: ATP5PF
• NCBI Official Synonym Symbols: F6; CF6; ATP5; ATPM; ATP5A; ATP5J
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• UniProt Gene Name: ATP5J
• UniProt Synonym Gene Names: ATP5A; ATPM; ATPase subunit F6