Standard Curve (Sample)
(Human CPM PicoKine ELISA Kit standard curve)
Human CPM ELISA Kit | CPM elisa kit
Human CPM ELISA Kit PicoKine
Reactivity
Human
Applications
ELISA
Synonyms
CPM; N/A; Human CPM ELISA Kit PicoKine; CPM elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CPM
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Applicable Applications for CPM elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CPM PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CPM PicoKine ELISA Kit standard curve)
Related Product Information for CPM elisa kit
Background: Carboxypeptidase M (EC 3.4.17.12, CPM) is an enzyme.The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.
Principle of the Assay: The Picokine™ Human CPM Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CPM with a 96-well strip plate that is pre-coated with antibody specific for CPM. The detection antibody is a biotinylated antibody specific for CPM. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human CPM protein as standards. To measure Human CPM, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CPM in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CPM in the sample.
Principle of the Assay: The Picokine™ Human CPM Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CPM with a 96-well strip plate that is pre-coated with antibody specific for CPM. The detection antibody is a biotinylated antibody specific for CPM. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human CPM protein as standards. To measure Human CPM, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CPM in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CPM in the sample.
Product Categories/Family for CPM elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,514 Da
NCBI Official Full Name
carboxypeptidase M
NCBI Official Synonym Full Names
carboxypeptidase M
NCBI Official Symbol
CPM
NCBI Protein Information
carboxypeptidase M; renal carboxypeptidase; urinary carboxypeptidase B
UniProt Protein Name
Carboxypeptidase M
UniProt Gene Name
CPM
UniProt Synonym Gene Names
CPM
UniProt Entry Name
CBPM_HUMAN
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Product Notes
The Human CPM cpm (Catalog #AAA126727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126727 ELISA Kit recognizes Human CPM. AAA Biotech's CPM can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CPM cpm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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