Rabbit anti-Human Carboxypeptidase M Monoclonal Antibody | anti-CPM antibody

Recombinant Anti-Carboxypeptidase M Antibody, Rabbit Monoclonal

Cat. Num. AAA255365

• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A
• Synonyms: Carboxypeptidase M, Antibody; Recombinant Anti-Carboxypeptidase M Antibody, Rabbit Monoclonal; Carboxypeptidase M Antibody, Rabbit MAb; carboxypeptidase M; anti-CPM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 105
• Specificity: Human Carboxypeptidase M/CPM
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CPM antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Carboxypeptidase M/CPM Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M/CPM (rh Carboxypeptidase M/CPM).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CPM was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CPM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CPM rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa)

WB (Western Blot)

(Anti-CPM rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CPM antibody

Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.

References

Deddish PA. et al., 1990, J Biol Chem. 265 (25): 15083-9. Nagae A. et al., 1992, J Neurochem. 59 (6): 2201-12. Skidgel RA. et al., 1996, Immunopharmacology. 32 (1-3): 48-52. Deiteren K. et al., 2009, Clin Chim Acta. 399 (1-2): 24-39.

NCBI and Uniprot Product Information

• NCBI GI #: 38327526
• NCBI GeneID: 1368
• NCBI Accession #: NP_938079.1
• NCBI GenBank Nucleotide #: NM_198320.3
• UniProt Accession #: P14384; Q9H2K9; B2R800
• Molecular Weight: 50,514 Da
• NCBI Official Full Name: carboxypeptidase M
• NCBI Official Synonym Full Names: carboxypeptidase M
• NCBI Official Symbol: CPM
• NCBI Protein Information: carboxypeptidase M
• UniProt Protein Name: Carboxypeptidase M
• UniProt Gene Name: CPM
• UniProt Synonym Gene Names: CPM
• UniProt Entry Name: CBPM_HUMAN